Antibacterial efficacy of essential oil spray formulation for post-milking disinfection in dairy cows

Background and Aim: Mastitis is an essential issue in dairy cows. Post-milking teat dips can help reduce this problem, but they employ harsh disinfectants, many bacteria are becoming increasingly tolerant. This study aimed to investigate the antibacterial activity of clove, citronella, sweet basil oils against common bovine mastitis causative agents Staphylococcus aureus, Streptococcus agalactiae, Escherichia coli develop antiseptic post-milking spray for use Materials Methods: The vitro antimicrobial was determined by broth microdilution time-kill assays. Essential oil-based sprays were developed. bacterial eradication efficacy formulations assays their stability tested repeated freeze-thaw cycles. most effective formulation Results: minimum inhibitory concentrations bactericidal S. E. range 0.78–6.25 µL/mL. tests indicated that oils’ depended on concentration contact time. All three preparations showed good stability. citronella highest potency. In vivo testing, eradicated aerobic skin cows (99.9% or 3-log10 reduction) within 1 min, which non-inferior a standard 0.54% iodine solution dip. Conclusion: Clove, vitro. Of these, oil promising be developed as with high excellent properties vivo. Keywords: activity, oils, disinfection, formulation.